Introduction to Genetic Analysis 416

Introduction to Genetic Analysis 416 - on File 44200...

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Summary 27 44200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 27 Application File on File Transcription Cam R 2 m ori 2 m GAL4- binding domain (BD) amp R TRP 1 + LEU 2 + Unite “Bait” protein GAL4 activation domain (AD) “Target” protein Interaction Target Bait Reporter LACZ GAL4 promoter GAL4 BD GAL4 AD Yeast two-hybrid vectors Figure 12-29 The yeast two-hybrid system for detecting gene interaction. The system uses the binding of two proteins under investigation to restore the function of the GAL4 protein, which activates a reporter gene. Cam, TRP, and LEU are components of the selection systems for moving the plasmids around between cells. The reporter gene is LACZ, which resides on a yeast chromosome (shown in blue). that is fused downstream of the GAL4 initiation re- gion. The two-hybrid system can be automated to make it possible to hunt for protein interactions throughout the proteome. Genomics and all the other “omics” have spawned
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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