Problems2944200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 29Application Fileon Filec.How many map units separate the three RFLPloci?SolutionThis is a mapping problem, but with the twist that someof the markers are traditional types (which we have en-countered in preceding chapters) and others are molecu-lar markers (in this case, RFLPs). Nevertheless, the prin-ciple of mapping is the same as that which we usedbefore; in other words, it is based on recombinant fre-quency. In any recombination analysis, we must be clearabout the genotype of the parents before we can classifyprogeny into recombinant classes. In this case, we knowthat the Oak Ridge parent must contain all O alleles,and Mauriceville all M alleles; therefore, the parents areal 1O2O3O 3al11M2M3Mand knowing this makes determining recombinantclasses easy. We see from the data that the parentalclasses are the two most common (34 and 36). We firstof all notice that the alalleles are tightly linked to RFLP
This is the end of the preview. Sign up
access the rest of the document.