Introduction to Genetic Analysis 419

Introduction to Genetic Analysis 419 - 44200 GRIFFITHS...

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44200 30 Chapter 12 Genomics 44200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 30 Application File 5. In sequencing a 1-gigabase genome with fivefold cov- erage by using BACs, how many clones would you be handling? 6. Fingerprints of three cloned inserts labeled P, Q, and R are obtained. Clone P has no bands in common with clone Q but has two bands in common with clone R. Q has three bands in common with R. What is the order of these three inserts in the genome. Show how they overlap. 7. What is the reason for choosing a set of clones that represents a minimal tiling path? 8. How would you subclone a BAC clone? Why is it necessary for genome sequence assembly? 9. What is the difference between a contig and a scaf- fold? 10. Two particular contigs are suspected to be adjacent, possibly separated by repetitive DNA. In an attempt to link them, end sequences are used as primers to try to bridge the gap. Is this approach reasonable? In what situation will it not work? 11. Individual sperm can be tested for certain markers by using PCR. A man heterozygous for a microsatellite
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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