44200 30Chapter 12 •Genomics44200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 30Application File5.In sequencing a 1-gigabase genome with fivefold cov-erage by using BACs, how many clones would you behandling?6.Fingerprints of three cloned inserts labeled P, Q, andR are obtained. Clone P has no bands in commonwith clone Q but has two bands in common withclone R. Q has three bands in common with R. Whatis the order of these three inserts in the genome.Show how they overlap.7.What is the reason for choosing a set of clones thatrepresents a minimal tiling path?8.How would you subclone a BAC clone? Why is itnecessary for genome sequence assembly?9.What is the difference between a contig and a scaf-fold?10.Two particular contigs are suspected to be adjacent,possibly separated by repetitive DNA. In an attemptto link them, end sequences are used as primers totry to bridge the gap. Is this approach reasonable? Inwhat situation will it not work?11.Individual sperm can be tested for certain markers byusing PCR. A man heterozygous for a microsatellite
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.