Problems3144200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 31Application Fileon Filebecame geographically isolated from each other. Youdetect polymorphisms in this strain by allowing asingle sequence to act as primer for PCR. Thisby chance spans and amplifies various regions of thegenome, showing up as bands on a gel. (This is calledRAPD analysis, standing for randomly amplifiedpolymorphic DNA.) One primer amplified twobands in haploid strain 1 and no bands in strain 2.These strains were crossed, and seven progeny wereanalyzed. The results were as follows:a.Draw diagrams that explain the difference be-tween the parents.b.Explain the origin of the progeny and their rela-tive frequencies.c.Draw an example of a single tetrad from this cross,showing the amplified bands.24.A certain disease is inherited as an autosomal dom-inant N.It was noted that some patients carrychromosomal rearrangements that have in commonthe property of always having a chromosomalbreakpoint in band 3q3.1 on chromosome 3. Four
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