Problems3344200 GRIFFITHS FREEM Ch-12 First Pages Sen 12-10-2003 p 33Application Fileon FileRead 1: ATGCGATCTGTGAGCCGAGTCTTTARead 2: AACAAAAATGTTGTTATTTTTATTTCAGATGRead 3: TTCAGATGCGATCTGTGAGCCGAGRead 4: TGTCTGCCATTCTTAAAAACAAAAATGTRead 5: TGTTATTTTTATTTCAGATGCGARead 6: AACAAAAATGTTGTTATTa.Use these six sequence reads to create a sequencecontig of this part of the H. sapiens genome.b.Translate the sequence contig in all possible read-ing frames.c.Go to the BLAST page of NCBI (http://www.ncbi.nlm.nih.gov/BLAST/ and see Appendix B) and see if you can identify the gene of which thissequence is a part by using each of the reading frames as a query for protein–protein comparison(BLASTp).32.A Neurosporageneticist wanted to clone the genecys-1,which was believed to be near the centromereon chromosome 5. Two RFLP markers (RFLP1 andRFLP2) were available in that vicinity, and so hemade the following cross:Oak Ridge cys-13Mauriceville cys-11(See Solved Problem 1 for an explanation of the OakRidge and Mauriceville strains.)
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