Unformatted text preview: 439 13.3 Transposable elements in eukaryotes insert transgenes. The P element in Drosophila provides one of the best examples of how geneticists exploit the properties of transposable elements in eukaryotes. USING P ELEMENTS TO TAG GENES FOR CLONING P elements can be used to create mutations by insertion, to mark the position of genes, and to facilitate the cloning of genes. P elements inserted into genes in vivo disrupt genes at random, creating mutants with different phenotypes. Fruit flies with interesting mutant pheno- types can be selected for cloning of the mutant gene, which is marked by the presence of the P element. The interrupted gene can be cloned with the use of P-element segments as a probe, a method termed trans- poson tagging. Fragments from the mutant gene can than be used as a probe to isolate the wild-type gene. USING P ELEMENTS TO INSERT GENES Gerald Rubin and Allan Spradling showed that P-element DNA can be used as an effective vehicle for transferring donor genes into the germ line of a recipient fly. They devised theinto the germ line of a recipient fly....
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.
- Spring '08