449 Problems PROBLEMS B ASIC P ROBLEMS 1. Suppose that you want to determine whether a new mutation in the gal region of E. coli is the result of an insertion of DNA. Describe a physical experi-ment that would allow you to demonstrate the pres-ence of an insertion. 2. Explain the difference between the replicative and the conservative modes of transposition. BrieFy de-scribe an experiment demonstrating each of these modes in prokaryotes. 3. Describe the generation of multiple drug-resistance plasmids. 4. BrieFy describe the experiment that demonstrates that the transposition of the Ty1 element in yeast takes place through an RNA intermediate. 5. Explain how the properties of P elements in Drosophila make gene-transfer experiments possible in this organism. 6. Nobel prizes are usually awarded many years after the actual discovery. ±or example, Watson, Crick, and Wilkens won the Nobel Prize in Medicine or Physiology in 1962, almost a decade after their dis-covery of the double helical structure of DNA. However, Barbara McClintock was awarded her
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.