Introduction to Genetic Analysis 464

Introduction to Genetic Analysis 464 - 463 14.2 Spontaneous...

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Unformatted text preview: 463 14.2 Spontaneous mutation same relative positions as those of the colonies on the original master plate. As expected, rare resistant mutant colonies were found on the replica plates, but the multi- ple replica plates showed identical patterns of resistant colonies (Figure 14-17). If the mutations had occurred after exposure to the selective agents, the patterns for each plate would have been as random as the mutations themselves. The mutation events must have occurred before exposure to the selective agent. Velvet surface (sterilized) Then pressed on replica plate that distinguishes wild and mutant genotypes Handle Pressed on master plate containing grown colonies Figure 14-16 Replica plating. Replica plating reveals mutant colonies on a master plate through their behavior on selective replica plates. [From G. S. Stent and R. Calendar, Molecular Genetics, 2d ed. W. H. Freeman and Company, 1978.] Replica plating Series of replica plates containing high concentrations of T1 phage and four Ton...
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This note was uploaded on 01/10/2011 for the course BIOL BIOL taught by Professor Johnson during the Spring '08 term at Aberystwyth University.

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