EBME_410_molecular_imaging

EBME_410_molecular_imaging - In Vivo Cellular and Molecular...

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Page 1 In Vivo In Vivo Cellular and Molecular Cellular and Molecular Imaging using MR and Bioluminescent Imaging Bioluminescent Imaging David L. Wilson Departments of Biomedical Engineering Departments of Biomedical Engineering and Radiology and Radiology Case Western Reserve University 2 Applications in Humans Applications in Humans Detection of disease Staging of disease Image guided therapies Assessment of therapy 3 The New Patient 23 million laboratory mice in 1998 Some estimate 100,000 currently at Case 1,000 hospital beds at University Hospitals of Cleveland 4 Applications in Mice Applications in Mice Develop methods for disease detection, staging, and assessment of therapy Phenotype genetically modified mice Characterize mouse models of disease; e.g., cancer Assess of drug and gene therapies Use as a tool to discover biology Etc.
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Page 2 6 Image Gene Expression Image Gene Expression Nature Medicine 4(2):245-247 7 NIH: In Vivo Molecular Imaging NIH: In Vivo Molecular Imaging Does not actually reveal molecules Detects signals indicating the presence of biochemical activity Functional because the processes are active and constantly changing Revolutionize small animal studies of phenotyping, disease detection, molecular therapeutics, preventative agents, gene therapies, and cell based therapies Will revolutionize the diagnosis, staging, and assessment of human diseases 8 Case Small Animal Imaging Facility $21M Investment: In Vivo Cellular and Molecular Imaging! 9 EBME 462: Spring EBME 462: Spring In Vivo Cellular and Molecular Imaging
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Page 3 10 References References C. Bremer, R. Weissleder. In Vivo Imaging of Gene Expression: MR and Optical Technologies. Acad Radiology Vol. 8, p15-23, 2001. B. Rice, M. Cable, M. Nelson. In vivo imaging of light-emitting probes. Journal of Biomedical Optics, Vol. 6, No. 4, p. 432-440, Oct. 2001. R. Weissleder, A. Moore, U. Mahmood, et al. In vivo magnetic resonance imaging of transgene expression. Nature Medicine, Vol. 6, No. 3, p351-354, Mar 2000. A. Louie, M. Huber, T. Meade. In vivo visualization of gene expression using magnetic resonance imaging. Nature Biotechnology, Vol. 18, p321-325. Mar 2000. K. Robinson. Contrast-enhanced MRI captures gene expression. Biophotonics International, p46-49, June 2000. Molecular Imaging. The American College of Radiology, Vol. 56, No. 6, p25-29, 2000. J. Bulte, S. Zhang, P. Gelderen, et al. Neurotransplantation of magnetically labeled oligodendrocyte progenitors: Magnetic resonance tracking of cell migration and myelination. Proc. Natl. Acad. Sci. Vol. 96, No. 26, 15256-15261, Dec 1999. 11 Outline Outline Definition and concepts Bioluminescent imaging methods and applications Fluoresent proteins MR contrast mechanisms MR imaging techniques Caged gadolinium Paramagnetic nanoparticles Summary 12 Image Gene Expression Image Gene Expression Nature Medicine 4(2):245-247 13 Gene Expression Gene Expression Gene expression: synthesis of protein from a gene All cells in an organism have copies of the genome.
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EBME_410_molecular_imaging - In Vivo Cellular and Molecular...

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