Plasmid Restriction Digest Presentation

Plasmid Restriction Digest Presentation - Plasmid...

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Click to edit Master subtitle style Plasmid Restriction Digest Pinal Patel Whitney Ridings Caroline Kim Melanie Kee December 1, 2010
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1/17/11 Plasmid Restriction Digest Our company maintains stocks of different bacteria that it uses to produce enzymes and proteins. The technician did not keep track of these stocks. Now it is our job to determine the identify of some unknown stocks of bacteria by observing the differences in their DNA. Strains of bacteria can potentially be distinguished from one another by looking for differences in the plasmid (a small, circular, double- stranded DNA molecule) they contain using the method of RFLP, Restriction Fragment Length Polymorphism . Our company has restriction maps for three of the plasmids of stocks. A bacterium with plasmid enclosed showing chromosomal DNA and plasmids.
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Click icon to add clip art How is this done? The plasmid is unique to each bacteria and contains a unique sequence of base pairs. Digestive enzymes cut the plasmids at certain
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This note was uploaded on 01/16/2011 for the course BIO 1107 taught by Professor Devartanian during the Spring '08 term at University of Georgia Athens.

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Plasmid Restriction Digest Presentation - Plasmid...

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