DNA Restriction Analysis

DNA Restriction Analysis - DNA Restriction Analysis of...

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DNA Restriction Analysis of unknown plasmid sample Pinal Patel TA: Joseph LaMattina November 17, 2010
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Introduction: This experiment is to perform restriction digests to determine the identity of an unknown plasmid. Strains of DNA can potentially be distinguished from one another by looking for differences in the plasmids they contain. One method to do this is Restriction Fragment Length Polymorphism (RFLP). In RFLP, DNA samples are cut into fragments using RE enzymes. Because they have different DNA sequences, plasmids can be distinguished from one another by digesting them with the same enzymes and comparing the number and size of the DNA fragments produced. From the study of Barionovi and other members, restriction fragment length polymorphism was used to determine the genetic relationship between a deviating Erwinia amylovora strain isolated from Amelanchier grown in Canada and other strains from Maloidaw and Rosoideae by DNA sequence analysis in representative strains (Barionovi, D, et al., 2006). Doctors and scientists can use DNA restriction analysis to discover an unknown bacterium or virus. This process is known as “Single Genome Fingerprinting,” and after identifying the restriction sites in the virus, the doctors can use to the test to “allow rapid identification of
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DNA Restriction Analysis - DNA Restriction Analysis of...

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