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Plasmid Restriction Digest

Plasmid Restriction Digest - Plasmid Restriction Digest...

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Click to edit Master subtitle style Plasmid Restriction Digest Pinal Patel Whitney Ridings Caroline Kim Melanie Kee December 1, 2010
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Plasmid Restriction Digest Our company maintains stocks of different bacteria that it uses to produce enzymes and proteins. The technician did not keep track of these stocks. Now it is our job to determine to identify of some unknown stocks. Strains of bacteria can potentially be distinguished from one another by looking for differences in plasmid (a small, circular, double stranded DNA molecule) they contain using the method of RFLP, Restriction Fragment Length Polymorphism. Our company has restriction maps for three of the plasmids of stocks. A bacterium with plasmid enclosed showing chromosomal DNA and plasmids.
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How is this done? The plasmid is unique to each bacteria and contains a unique sequence of base pairs. Digestive enzymes cut the plasmids at certain restriction sites that contain a specific sequence of base pairs. This fragments the plasmid into multiple pieces with different lengths. The enzymes available
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