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Midterm+2+keys_SMB - Biological Sciences 101-002 Dr Mark...

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Biological Sciences 101-002 Dr. Mark Sanders and Dr. Siobhan Brady Fall Quarter 2010 Midterm II Exam Key FORM A 1a. 1b. (worth 11 pts., -1 each gene, insert, orientation or minute incorrect) 2. DNA Replication Transcription An RNA primer is required for synthesis X An enzyme other than polymerase is required to unwind the strands X Synthesis is in the 5’ to 3’ direction x X Binding to a DNA sequence can initiate the process x X An exact copy is made of the coding strand Synthesis requires formation of a phosphodiester bond x X Nucleotides have a 2’-OH in its sugar moiety X (RNA primer) or 0 accepted X 1 point for each correct answer 3a. 1 point each for leading and lagging strand 1 point for correct labeling of Okazaki fragment 1 point for correct labeling of RNA primers 1 point each for the correct labeling of direction for the copied DNA from the leading and lagging strand 1 point for correct positioning of replication fork
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3b. Eukaryote – proteins from the origin recognition complex bound. 1 point for eukaryote, and 1 point for the correct explanation. 3c. Coding Strand Template Strand Gene 1 Top Bottom Gene 2 Bottom Top 0.25 points for each correct answer in the table Gene 1 Transcribed Sequence (label the 5’ and 3’ end): 5’AUG CCA CGA CCA UAC AUU UAG3’ OR 5’AUG CCA GCA CCA UAC AUU UAG3’ 1 point for correct direction, 1 point for switching T to U, 1 point for correct copying Gene 2 Transcribed Sequence (label the 5’ and 3’ end): 5’AUG CAU GCC UAU AUC UAA3’ OR 5’AUG CAU GCC UCU AUG UUA 1 point for correct direction, 1 point for switching T to U, 1 point for correct copying 3d. Gene 1: Met-Pro-Arg-Pro-Tyr-Ile-(stop) OR Met-Pro-Ala-Pro-Tyr-Ile-(stop)
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