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Unformatted text preview: BPS3101 PRACTICE QUESTIONS #3 Fall 2010 QUESTION 1 Suppose that the human gene X encodes an enzyme which binds drug D through a domain encoded within exon 2. The gene X region is shown below: exons = black, introns = dotted lines; intron sequence = lower case letters; exon sequences = upper case letters with initiation and stop codons in underlined italics. Restriction sites are E: EcoRI, B: BamHI, H: HindIII. Individuals which have a G-to-T mutation within intron 1 (at position of black triangle), show alternative splicing such that exon 2 is skipped over. Describe two screening methods (one that is DNA-based and the other RNA-based) to determine whether an individual carries such a mutant allele for gene X and whether it is in a heterozygous or homozygous state. Show expected hypothetical results in clearly-labelled schematics. QUESTION 2 Suppose that the restriction map below shows bacterial gene A (black bar), which encodes a membrane-located transporter needed for drug M to enter the bacterial cell. Arrows show the positions of IS elements found in strains #1 to #4. Assume that only one IS element is present per positions of IS elements found in strains #1 to #4....
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This note was uploaded on 01/16/2011 for the course BPS 3101 taught by Professor Bonen during the Fall '09 term at University of Ottawa.
- Fall '09