10practset2answersweb

10practset2answersweb - Practice set#2 question 1 black...

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Practice set #2, question 1 black bars = exons so arrows show positions of UTRs Southern analysis Northern analysis B E H E+H B E H E+H ~2 kb 6 kb - 5 kb - 3 kb - 1 kb - Probe: exon 3 intron 2 Probe: exon 3 intron 2
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Practice set #2, Question 2 Oligomer specific hybridization: Isolate DNA from mice (i) to use as hybridization probe (tagged & denatured) on oligomer grid G A G A A T C T C C G A G A C C T C C T C G A G A A T C T C T G A G A C C T C C T C ..TC C GA. .. ...TC T GA. .. Hybridization conditions such that no stable hybrid formed if one mismatch GCTC G GAGA +/+ GCTC A GAGA 5’ . .. TCTC C GAGC. .. 3’ 5’ . .. TCTC T GAGC . .. 3’ -/- SNP detection GCTC G GAGA GCTC A GAGA On array, could use oligomer corresponding to either strand, because tagged DNA probe is double-stranded (prior to denaturation) + / - GCTC G GAGA GCTC A GAGA Note: many identical DNA (or oligomer) molecules present in experiments (even though figures in texts may show as single)
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OR Isolate DNA from mice (ii) attach denatured DNA samples to 2 separate
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10practset2answersweb - Practice set#2 question 1 black...

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