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10practset3answers - Practice set#3 Question 2 150 bp 200...

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Practice set #3, Question 2 200 bp 300 bp 250 bp 500 bp E S S S S E #1 # 4 550 bp E S S S S E #2 500 bp E S S S S E Response to drug M Wild type (no IS in gene A) Sensitive Strain with IS at position #1 Resistant Strain with IS at position #2 Resistant Strain with IS at position #3 Resistant Strain with IS at position #4 Sensitive wt #1 150 bp 50 bp 600 bp 500 bp 400 bp 300 bp 200 bp 100 bp 50 bp Southern probe = gene A #4 Model: inactivation of gene A if there is an IS element in promoter region or within coding sequence except not if within C-terminal coding region Transposon mutagenesis provides information about protein structure/function
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