10questions2 - BPS3101 PRACTICE QUESTIONS#2 FALL 2010...

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BPS3101 PRACTICE QUESTIONS #2 FALL 2010 QUESTION 1 You have sequenced the region of an insect genome containing gene X, shown on the restriction map below (black bars = exons, dotted lines = introns). Arrows show the positions of short stretches of that sequence, with potential initiation and termination codons in underlined italics. Restriction sites are E: EcoRI, B: BamHI, H: HindIII. a) Show what you would expect to see if exon 3 were used as a hybridization probe in (i) northern analysis and (ii) Southern analysis - BamHI (lane 1), EcoRI (lane 2), HindIII (lane 3) EcoRI+HindIII (lane 4). Be sure to indicate size markers on your figures. Would your answer differ if intron 2 were used as a probe? Give your rationale. For extra practice, design primers to generate an RT-PCR product for gene X and give its size(s). If it were used as a probe in (i) a Southern hybridization experiment (as described above) or (ii) a northern experiment what would you expect to see?
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