10test1answersweb - Question 1c E 550 bp RFLP analysis A...

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Question 1c RFLP analysis these fragments will be same So if PCR amplify just blue region & then restrict with enzyme A & run on gel... we can ignore other restriction fragments E E A A A E E 550 bp 420 bp 170 bp 800 bp 250 bp 750 bp same in any rabbit Homozygous (A site present) Homozygous (A site absent) Heterozygous Alternatively, digest rabbit DNA, run on gel for Southern hybridization where probe is region of polymorphic site (or whole region shown in schematic) 1050 bp 800 bp 250 bp
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ATG 3’ Gene X Gene Y AAT TAG GTA 5’ 5’ 3’ mRNA mRNA 5’ 5’ 3’ 3’ Question 2b One possible arrangement: or ATG Gene X Gene Y TAG 5’ 5’ 3’ 3’ AAT GTA 5’ 3’ 3’ 5’ mRNA Normally, bacterial mRNAs have short UTRs and do not overlap with other genes RNAs from other genes ... but if 3’ UTR of gene X mRNA were to extend into coding region of gene Y, amplicon would contain gene B sequence ....
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