BCH3356 Final Exam marking scheme

BCH3356 Final Exam marking scheme - BCH3356 Molecular...

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BCH3356 : Final Exam, page 1/14 BCH3356 Molecular Biology Laboratory Final exam: This exam is worth 30 % of the final mark for the course BCH3356 Name:_______________________ Student number:_______________ Instructions All questions should be answered within the space provided on THIS COPY EXAM. You may use the endorsement of pages for your draft calculations. Students are allowed to use a calculator. Proctors will not answer any question during the exam.
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BCH3356 : Final Exam, page 2/14 Part I ( /30 marks): Underlying principles of molecular techniques 1. BCH3356 Crossword (10%) ACROSS Before proceeding with ligation, pTrcHisB was treated with a PHOSPHATASE. Type of membrane used for the electrophoretic transfer: PVDF Restriction enzymes are also called restriction ENDONUCLEASES The chemical added to a liquid culture to promote the overexpression of your recombinant protein was IPTG A negative control is commonly used to assess the BACKGROUND signal. The discrimination capacity of a test in detecting a given signal: SPECIFICITY The transfer of a cloned fragment of DNA from one vector to another: SUBCLONING The ion used to recharge the affinity chromatography column used to purify your recombinant T7 RNA polymerase : NICKEL The ligation strategy used in BCH3356 is considered as DIRECTIONAL. DOWN Number of steps per PCR amplification cycle: THREE The plasmid conformation that moves the faster on a gel is the SUPERCOILED conformation. Process of DNA melting: DENATURATION The name of the destination vector used for the ligation you performed is pTRCHISB. pH conditions used for the miniprep: ALKALINE Component of the lac operon that is constitutively expressed in host cells transformed with an empty or recombinant pTrcHisB: REPRESSOR PCR product: AMPLICON Is the primer that anneals onto the antisense DNA strand: FORWARD Ability of a cell to take up extracellular DNA: COMPETENCY Reformation of complementary strands of DNA: ANNEALING 1. 0.5 was subtracted for every missing or wrong answer.
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BCH3356 : Final Exam, page 3/14 2. Identify and explain the main procedural steps for the minipreparation of plasmid DNA by alkaline lysis. Molecular details are to be emphasized. Use a separate paragraph to describe each major step. (10%) 3 MARKS: Cell lysis with NaOH for denaturation of plasmid and chromosomal DNA 3 MARKS: Neutralization with K acetate: plasmid DNA reanneals faster than chromosomal DNA because the two strands of the plasmid DNA remain entangled due to supercoiling. 4 MARKS: Centrifugation to pellet chromosomal DNA and other aggregates including denatured proteins. Plasmid DNA is found in the supernatant. 3.
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This note was uploaded on 01/16/2011 for the course BCH 3356 taught by Professor Odette during the Fall '08 term at University of Ottawa.

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BCH3356 Final Exam marking scheme - BCH3356 Molecular...

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