quiz-2_key - QUIZ 2 BIS-101D Fall 2009 A segment of a...

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QUIZ 2 BIS-101D Fall 2009 A segment of a polypeptide chain is Arg-Gly-Ser-Phe-Val-Asp-Arg. It is encoded by the following segment of DNA. Arg Asp Val Phe Ser Gly Arg 3’ GGC-TAG-CTG-C TT-CC T-TGG-GGA 5’ nontemplate 5’ CCG-ATC-GAC-GAA-GGA-ACC-CC T 3’ template Which strand is the template strand? Label each strand with its correct polarity (5’ and 3’). (2) (a) Using the codon table, show the consequences on subsequent translation of the addition of an adenine base to the beginning of the following coding sequence. Arg Ser Glu Pro Arg Asp Lys His CGA-UCG-GAA-CCA-CGU-GAU-AAG-CAU Thr Ile Gly Thr Thr Stop ACG-AUC-GGA- ACC- ACG-UGA-UAA-GCA-U Translation is prematurely terminated. (b) The insertion of the adenine in the above example causes what type of mutation? A frameshift mutation
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2 (3) Find glycine codons in the codon table. Isoaccepting tRNA gly have anticodons shown below. Using wobble rules, show how these two anticodons recognize the four glycine codons during the translation process. Indicate 5' and 3' end for each codon and
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quiz-2_key - QUIZ 2 BIS-101D Fall 2009 A segment of a...

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