Biol110-10-Midterm Review

Biol110-10-Midterm Review - Biol110-10-Review for the...

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Lecture 1- Introduction 1) Why is it advantageous to choose the experimental organism or cell type according to the cell biological question being asked? 2) What is the evidence indicating that mitochondria and chloroplasts are of bacterial origin and became symbions with eukaryotes during evolution? 3) How do you suppose bacteria secrete proteins if they don’t have a membranous secretory pathway? 4) What is the best combined approach to study a particular cell biological process? Lecture 2- Methods 1) See additional methods text posted. Learn what each technique can generally do from reading the figure titles. Biol110-10-Review for the Midterm Exam
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Lecture 3- Membranes 1) What is the main function of biological membranes and how can you explain this function at the molecular level? 2) Why are membranes referred to as a Fluid Mosaic? What does this mean at a molecular level? 3) What are the permeability properties of biological membranes? 4) What determines the geometry of phospholipid molecules? Why are different geometries important? 5) What is meant by lipid rafts? 6) What three molecular features in phospholipid molecules govern membrane fluidity and appearance (i.e thickness)? 7) Where are phospholipids synthesized and in what three ways are they moved around the cell? 8) Why are detergents useful in cell biological research? What are some of their applications? 9) How does water move through biomembranes and what controls the net directionality of its movement? Explain at a molecular level. How do cells handle this osmosis issue?
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Lecture 4- Porins (and other membrane associated proteins) 1) How can proteins be tethered to biological membranes? 2) How can you predict if a particular protein is likely to associate with membranes? 3) How can you test whether a particular protein is mobile in membranes? 4) How can proteins be immobilized on membranes to perform a local function? 5) How is transport of non-membrane permeable substances across biomembranes achieved and what governs the net direction of particle movement? 6) What dictates the selectivity of channel proteins? 7) What are signal-activated channels and what signals activate them? 8) What is the difference between simple diffusion, facilitated diffusion and active transport? What forms of energy can drive active transport? 9) What are coupled-carriers and why are they important at a cellular level? 10) How can you record the activity of ion channels? What are ionophores? 11) How can you study the function properties of a channel protein in isolation? 12) How can you determine the topology of proteins on membranes?
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Lecture 5- Membrane Biogenesis and Mitochondrial translocons 1) How are proteins targeted specifically to distinct cellular locations? 2)
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This note was uploaded on 01/17/2011 for the course BIOL 110 taught by Professor Rexach during the Fall '10 term at UCSC.

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Biol110-10-Midterm Review - Biol110-10-Review for the...

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