Biol110-10-Problem Set#5

Biol110-10-Problem Set#5 - COPII coat subunits How do COPII...

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Student number__________ Grader number___________ Biol110-Cell Biology Fall 2010 Problem Key #5 Question #1- Vesicle-Mediated Transport in Cells How do lumenal and integral membrane proteins that function in the ER retain their localization during their lifetime despite the fact that they occasionally leave the ER in vesicles by mistake? Explain for each type. (5 pts) Recently homologues of COPII coat subunits were identified in cells, which can substitute for Sec24 in formation of COPII coated vesicles. What do you think is the functional significance of these alternate
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Unformatted text preview: COPII coat subunits? How do COPII vesicles loose their coat in preparation for fusion with the cis-Golgi? (5 pts) Based on the topology of v- and t-SNAREs in the membrane (they have a short lumenal C-terminus), why is it important that v- and t-SNAREs make parallel coiled coil interactions during vesicle fusion? Explain. What would happen if they made anti-parallel interactions? (5 pts) What is the role of NSF and SNAPs in vesicular transport? Why is ATP hydrolysis needed? Why do vesicles accumulate in the absence of NSF activity? (5 pts)...
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