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Lecture6 - 1 Structure determination"Imaging macromolecules...

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1 Structure determination: "Imaging" macromolecules X-ray diffraction of protein crystals -electron density and modeling building -structured vs. unstructured regions Nuclear Magnetic Resonance -measuring interactions between "nearby" nuclei -distant constraints -ensemble structures Electron Microscopy -imaging molecules with electrons -3D reconstruction -electron density
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2 Different strengths and weaknesses of each technique X-ray crystallography -high resolution -it is hard to crystallize many proteins -need large amounts of sample -"static" snapshot Nuclear Magnetic Resonance -allows dynamic measurement -versatile applications -on applicable to "small" proteins -need large amounts of sample Electron Microscopy -need only tiny amounts of sample -the bigger the molecule the better -cannot apply to "small" proteins -limited resolution
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3 Crystal -> Diffraction pattern -> Electron density -> Model Overview of X-ray Crystallography
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4 A brief introduction to "imaging" with electromagnetic waves Blind man bouncing balls off of a statue: More detailed information by using: A. basketballs B. tennis balls C. golf balls D. peas
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