ProblemSet4

ProblemSet4 - BIOC100A Problem Set 4 1 a In the...

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BIOC100A Problem Set # 4 1. a. In the illustration on the right, do you think the illustrator was trying to depict A-DNA or B-DNA? Give your reasoning. What is wrong with the image? b. Given what you think the illustrator was trying to illustrate, what major error(s) was (were) made? 2. Certain enzymes can "nick" DNA, which means to cleave the phophate backbone of one strand of DNA in a double helix. How would this affect the topology of: a. a linear strand of DNA? b. a relaxed plasmid? c. a supercoiled plasmid 3. Shown to the right is a plasmid purified from bacteria run on an agarose gel with a DNA stain (ethidium bromide) . The gel separates molecules based on their size/charge ratio. Lane 1 is purified plasmid with no other contaminating DNA. Lane 2 is the same plasmid cut at one position within the plasmid with a restriction endonuclease. Propose a hypothesis for the identity of the three different bands that appear on the gel. 4. Exposure of DNA to sodium nitrate can deaminate adenine bases causing some of the
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This note was uploaded on 01/17/2011 for the course BIOC 100A taught by Professor Harrynoller during the Fall '10 term at UCSC.

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ProblemSet4 - BIOC100A Problem Set 4 1 a In the...

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