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Quiz 4 - Lightr Can m In Hu— 4 r-rr any Bio 221 Name...

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Unformatted text preview: Lightr Can. m. In: Hu— 4. r.,-rr any Bio 221 Name Keg (Please PRINT clearly) Quiz 4 1. What equation would you use to solve the following problem: 7 How long would it take to reach stationary phase in a liter of culture (1012 cells) inoculated with a single colony (105 cells), if the bacterial growth rate is 1.2 per hour? A. 1.2=(1012/105)ex N= ,\l a” B. 105=(10‘2) (1.2x) ‘ 5 C. 1012: 1.2xe10 IO“ - ro‘e D. 1012 = 105 e” x / 5 ‘ E. 1012/1.2=e10 X M .4», le all; /‘ 2_. Which of the following would be considered a Viable Cell Count method? i A. Most Probable Number method L. WU» M 32:0 (H . B. Petroff-Hausser Chamber - (brat We ( Lu—rémé} C. Turbidity (OD) measurement - Ame! w“. mi.» lrg ht, 4w . D. Coulter Counter - direct M (M Wu» ,~,,J.m~,r; t4». flaw/Jaw) E. Weighing the cell pellet from a bacterial culture - 59% [M ,, M WUL A.» m». 3. The decimal reduction time for disinfecting a Staphylococcus-contaminated steel countertop with 500 ppm chlorine bleach is 2 minutes. Which of the following would decrease the decimal reduction time? (9.4-le -— (u) lot) ’l place: 6. “A1..." 2. e mew— rm. it. swarm: ,. My»: ’7 A. using a quaternary ammoni deter ent rather than chlorine bleach /‘ B. disinfecting a rough surfa e such as a sieve or screen instead of smooth 5 e1 C. disinfecting a greasy surface such as a dish drain instead of a countertop D. disinfecting a suxtgce contaminated with Mycobacteria rather than Staphylococcus E. using 5000 ppm hlorine bleach rather than 500 ppm—fl "M‘i “‘ id" (“"7 {”7”} damkd‘zmi‘: Hut/(4 [L53 'v-l’kdm— with 5m. 4. A mouthwash called “Hexodent” lists the following ingredients: 8% alcohol, 0.2% u—r chlorhexidine, mint flavor. How does this mouthwash kill bacteria? f 2 "wt, 60—232. I not 7% A. The alcohol denatures proteins. B. The alcohol damages DNA. --9 W 4"“ f ”‘9'“ M ”“7 ‘1. ("W7 W C. The chlorhexidine dissolves cell membranes. L d d» M D. The chlorhexidine cross-links and rigidities proteins. A 01‘“ V ‘5 E. hlorhexidine + ethyl alcohol —> ethylene oxide, which oxidizes DNA. ”MMAL rude-v»; Ema“ cute mum‘s 4., new ‘7 4 ”“1““ CM 5. Which of the following correctly describes a non-competitive enzyme inhibitor? 3“ ~ A. It binds to the active site of the enzyme. / “7,9 at ”A; B. Even very low concentrations of it caninhibit an enzyme. iii-av. C. It must at least partially resemble the structure 0 t enz me’s normal substrate. "' D. It must be a product of the reaction catalyzed by the enzyme. E. It attaches Wm the enzyme. \ M~'H\.r 67/“, M31: In 4 {mm 8. MM fi/w cut-(ulna “mtg/(rt? flea m wt. in WwMWkal’l Ii it‘s A ...
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