Exam3 Fall 2010KEY

Exam3 Fall 2010KEY - Bio 221 Fall 2010 Name_Key Exam 3[TEAR...

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Bio 221 Fall 2010 Name__________ Key ______________________ Exam 3 [TEAR OFF THE FIRST PAGE OF THE EXAM AND HAND IT IN WITH THE SCANTRON.] Short Answer. 25 points (5 points each). 1. Fill out the following table, showing how T C and T H cells differ. T H T C Major surface protein CD___ 4 ____ CD___ 8 ____ Recognizes antigens from phagolysosome (or exogenous) what part of the APC? MHC-II (+0.5 for these answers here) MHC-I Recognizes antigens MHC-II MHC-I displayed on . . . (also accept: APC any nucleated cell) Major product of this type cytokines perforin of T cell Main role of this type of stimulating B-cells, Macrophages T cell in immunity and other T-cells 2. Obviously, many bacterial diseases are caused by infection with the pathogenic organism. But some are also caused by intoxication and by your body's immune response to the pathogen. Explain how these last two mechanisms can cause disease , and name one disease caused by each of them . intoxication: The bacterium doesn’t have to be present in the body, but produces a toxin that can be ingested or absorbed to cause disease by damaging cells or membranes or triggering toxemia in the host Some examples: anthrax, tetanus, toxic shock syndrome, diphtheria (many others) immune response: Your body’s immune system responds to the presence of a microorganism in your body, causing inflammation, or producing antibodies against the disease. The inflammation can damage your own tissues, or in cases of immune mimicry the antibodies can direct ADCC against your own cells, causing cell death and more inflammation. Some examples: tuberculosis, PID, rheumatic fever, glomerulonephritis, pneumonia (many others) cytoplasm (or endogenous) killing infected cells
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2 3. You want to use a Western Blot as an indirect immunologic test. Either list or sketch the steps you would need to carry out in order to perform this test. (Note: If you don't recall what a Western Blot is, you can get 4 of the 5 points for this question by listing or sketching the general steps involved in an indirect immunologic test.) Starting with Western Blot: (+2 for using known antigen to detect antibody, +1 for electrophoresis) 1) Separate known antigens by electrophoresis and transfer to membrane 2) Add patient’s serum (antibodies) to membrane Starting with indirect immunologic test: (+2 for using known antigen to detect antibody) 1) Add known antigens to slide or microtiter well 2) Add patient’s serum (antibodies) to slide or well For all: (+2 for secondary Ab and detection) 3) Add secondary Ab that recognizes Fc part of human IgG and is linked to a dye or an enzyme 4) Detect the second Ab by adding substrate, exposing to film, looking for fluorescence, etc. 4.
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Exam3 Fall 2010KEY - Bio 221 Fall 2010 Name_Key Exam 3[TEAR...

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