Biosensors - Biosensors and Bioreporters Reasons for using a biological sensor P env Reporter Gene Requirements 1 Specific

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Biosensors and Bioreporters
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Reasons for using a biological sensor:
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Reporter Gene P env Requirements: 1) Specific environmentally-responsive promoter 2) Reporter gene appropriate for the environment being interrogated Reporter gene should be rare in environment Gene function should be specific Should be highly sensitive to levels of transcription
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β-galactosidase encoded by lacZ X-gal
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Easily measured: Chromogenic substrate - x-gal cleaved to produce blue compound Fluorescent substrates also available Commonly used in lab assays Problems: Not very sensitive - > 10 7 cells/sample needed Pigments and particulates in sample interfere with signal Plants and soil microbes have indigenous B-gal activity - high background lacZ encoded B-galactosidase
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lux genes - encode bioluminescence Found in a variety of bioluminescent bacteria Catalyze chemical light production reaction
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LuxCDABE-encoded bioluminescence attractive reporter for several reasons: Light easily detected and quantified 1) Photographic films 2) Light-sensitive cameras 3) Photon-counting devices Relatively sensitive - 10
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This note was uploaded on 01/19/2011 for the course ESPM 192 taught by Professor Lindow during the Fall '10 term at University of California, Berkeley.

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Biosensors - Biosensors and Bioreporters Reasons for using a biological sensor P env Reporter Gene Requirements 1 Specific

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