ESPM 192 DNA markers II, conservation 2010

ESPM 192 DNA markers II, conservation 2010 - Review from...

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Unformatted text preview: Review from last time: ask yourself for each type of marker: What is the variation that is being measured, exactly? Why does this marker vary in nature? How does this type of marker evolve ? How variable or polymorphic is the marker ? What are some uses for the marker ? --many, examples: invasive species, conservation, forensics, food security, etc. Why are some genetics markers good for some purposes and others for other purposes? Molecular Markers II, Conservation Biology George Roderick, Kate Stoeckle and Louisa Strauss DNA barcoding: 60 samples from 4 restaurants 10 markets in NY what Fsh was the sushi ? sequenced mitochondrial DNA 93% could be matched to fish species in database for fish DNA 23% of samples were mislabeled--White tuna (albacore) was Mozambique tilapia--Mediterranean red mullet was a Caribbean spotted goatfish--7 of 9 red snappers were mislabeled, including fish from SE Asia, South Pacific, Nile, Africa, and an endangered fish from N Atlantic (Stoeckle and Strauss 2008, Wong and Hanner 2008) Why care about cats? 1. Wild cats threatened by hybridization with domestic cats 2. Feral cats are a invasive species and a conservation issue, because they eat song birds 3. Previously thought that color (pelage) was diagnostic between wild and domestic cats 1. Examined 8 microsatellite loci 2. Hardy-Weinberg equilibrium across what was thought to be distinct wild and domestic cats-- what does this mean? 3. Pelages were somewhat different, but did not correspond to whether cats were wild or domestic 4. Implications: need to control domestic cats Note: cats are not dead Point: many hybrid cats [axis indicates shared alleles] Different data sets Genome scans to identify loci under selection (Storz 2005) Compare pattern...
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This note was uploaded on 01/19/2011 for the course ESPM 192 taught by Professor Lindow during the Fall '10 term at University of California, Berkeley.

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ESPM 192 DNA markers II, conservation 2010 - Review from...

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