ESPM 192 DNA markers II, conservation 2010

ESPM 192 DNA markers II, conservation 2010 - Review from...

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Unformatted text preview: Review from last time: ask yourself for each type of marker: What is the variation that is being measured, exactly? Why does this marker vary in nature? How does this type of marker evolve ? How variable or polymorphic is the marker ? What are some uses for the marker ? --many, examples: invasive species, conservation, forensics, food security, etc. Why are some genetics markers good for some purposes and others for other purposes? Molecular Markers II, Conservation Biology George Roderick, roderick@berkeley.edu Kate Stoeckle and Louisa Strauss DNA barcoding: 60 samples from 4 restaurants 10 markets in NY what Fsh was the sushi ? sequenced mitochondrial DNA 93% could be matched to fish species in database for fish DNA 23% of samples were mislabeled--White tuna (albacore) was Mozambique tilapia--Mediterranean red mullet was a Caribbean spotted goatfish--7 of 9 red snappers were mislabeled, including fish from SE Asia, South Pacific, Nile, Africa, and an endangered fish from N Atlantic (Stoeckle and Strauss 2008, Wong and Hanner 2008) Why care about cats? 1. Wild cats threatened by hybridization with domestic cats 2. Feral cats are a invasive species and a conservation issue, because they eat song birds 3. Previously thought that color (pelage) was diagnostic between wild and domestic cats 1. Examined 8 microsatellite loci 2. Hardy-Weinberg equilibrium across what was thought to be distinct wild and domestic cats-- what does this mean? 3. Pelages were somewhat different, but did not correspond to whether cats were wild or domestic 4. Implications: need to control domestic cats Note: cats are not dead Point: many hybrid cats [axis indicates shared alleles] Different data sets Genome scans to identify loci under selection (Storz 2005) Compare pattern...
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This note was uploaded on 01/19/2011 for the course ESPM 192 taught by Professor Lindow during the Fall '10 term at University of California, Berkeley.

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ESPM 192 DNA markers II, conservation 2010 - Review from...

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