Lect8-KR

Lect8-KR - Bacterial cell division PMB/MCB112 September 17,...

Info iconThis preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Bacterial cell division PMB/MCB112 September 17, 2010
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Bacterial shape is governed by the shape of the PG sacculus, which is made by PBPs. But how do the PBPs know what to do? Contrary to the assertions of older textbooks, bacteria CONTAIN CYTOSKELETAL PROTEINS and they are involved in generating bacterial shape. There are homologs of all three major cytoskeletal elements found in eukaryotes Actin = MreB, Mbl Tubulin = FtsZ Intermediate filaments = CreS (crescentin)
Background image of page 2
Caulobacter , E. coli and B. subtilis cells depleted of MreB lose their characteristic rod shapes and lyse
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
MreB polymerizes into filaments that resemble actin filaments in vitro In living cells, GFP-MreB and GFP-Mbl assemble into dynamic helical structures
Background image of page 4
Caulobacter MreB Bacillus subtilis Mbl
Background image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
So what’s the connection between PBPs and MreB? A = normal location of PBP2 in Caulobacter D = location of PBP2 when MreC is depleted E = location of PBP2 when MreB filaments are depolymerized by A22
Background image of page 6
MreBCD form a complex that positions the PG synthesis machinery along lateral cell walls MreD is TM, and MreC has TM spans plus periplasmic domain MreBCD are expressed together in an operon MraY makes Lipid I, MurG makes Lipid II, RodA may be flippase for Lipid II without MreBCD, PBP2 is mislocalized and can’t make normal lateral walls cells become fat lemons and lyse
Background image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Crescentin (CreS) is a homolog of intermediate filament proteins that is responsible for the curved shape of Caulobacter and other cells
Background image of page 8
What proteins act at the division plane to form the new septum?
Background image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 01/19/2011 for the course C 112 taught by Professor Ryan during the Fall '10 term at Berkeley.

Page1 / 22

Lect8-KR - Bacterial cell division PMB/MCB112 September 17,...

This preview shows document pages 1 - 10. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online