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1 Spectrophotometer & Green Food Color

1 Spectrophotometer & Green Food Color - Nick Duval Bio...

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Nick Duval 01/19/11 Bio 20L: Ogren Wed 1:00 – 5:00 Spectrophotometric analysis of green food color Objective: The object of the experiment was to identify different dyes and their concentrations used to make commercial green food coloring by means of absorbance readings and comparisons to known absorbance spectra. A spectrophotometer was used to calculate the absorbance levels of the dye at different wavelengths over the visible spectrum of light. Methods & Materials:All steps were followed as outlined in the lab manual (Ref. 7). The 0.2 mL of green food coloring was placed into a test tube where it was then supplemented with 1.8 mL of diluent, a pH buffer composed of NaPO 4 and a pH of 7.4. The pH buffer was required to not add additional charged components into the proceeding dilutions and to dilute the food color. The second dilution was placed in a second test tube composed of 0.2 mL of the first dilution and 1.8 mL of the pH buffer diluent. A third and final dilution was formed by pipetting 0.2 mL of the second dilution into a third test tube and 1.8 mL more of the pH buffer diluent. The contents of each
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