Lecture 5 Molecular Genotyping- PCR and DNA Sequencing

Lecture 5 Molecular Genotyping- PCR and DNA Sequencing -...

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1 Announcements Lecture 5 Hourly Exam is this Friday 20 multiple choice questions (Scantron) in 50 minutes Come to the exam knowing your assigned seat (website) and bring #2 pencils, erasers, and student ID We will provide the scantron cards The hourly exams from previous years are posted
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2 Dr Yi's Midterm1 Review Wednesday October 6 PCB 1100 from 7-9PM be sure to check the bio sci tutoring website https://eee.uci.edu/programs/biotutor/ and print the review packet before attending the review session.
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3 Studying for Exam Lecture 1 Definitions DNA is the genetic material Lecture 2 DNA structure DNA replication Lecture 3 Transcription Splicing Lecture 4 Translation Genetic code Lecture 5 PCR DNA sequencing Lecture 6 Germ versus Somatic cells Meiosis Focus on concepts Do your best! Active learning
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4 Crick-Brenner Experiment that demonstrated that the codon was a triplet Two types of mutations: (+) and (-) Single mutants do not grow, but double (+)(-) mutant can grow Assumed that each mutation was a single base pair frameshift: (+) is a single bp insertion and (-) is a single bp deletion The fact that the triple mutant (+)(+)(+) or (-)(-)(-) could grow (phenotype is normal) argues for a triplet code Phenotype + - + + + - - - - + (phage growth)
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5 Elucidation of Genetic Code Synthetic RNA molecules were made of a specified sequence (e.g. UUUUUUUUU) These RNA templates were translated using in vitro translation reactions No start or stop codons are needed in this in vitro reaction; translation can begin at any codon All three reading frames are translated into protein in this in vitro translation The amino acid composition of the resulting polypeptides were analyzed Deduced which amino acid corresponded to what codon
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This note was uploaded on 01/21/2011 for the course BIO SCI 65 taught by Professor Yin during the Spring '10 term at Saint Joseph's University.

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Lecture 5 Molecular Genotyping- PCR and DNA Sequencing -...

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