Chem110 Chromatography Lab Report

Chem110 Chromatography Lab Report - Identifying FD&C...

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George Fountain III Chemistry 110; Section 010 October 14, 2010 Lab Instructor: Yumi Lab Partner: Kelly Starosta
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Abstract: Chromatography is an experimental technique used to separate and identify components in mixtures involving a liquid/solid phase (stationary) or liquid/gas phase (mobile). When samples run up to the 1.5-cm mark of the paper, times were recorded for the lapsed time of each chromatography paper. The samples will each be averaged using their R.F. values, which is the net distance traveled by the individual component. The quickest solvent appeared to be the table salt, which came in one minute shorter than the water’s elapsed time by about one minute. After calculating the average R.F. value for each sample, the final results showed the Yellow Unknown contained the colors Red 3 and Yellow 5, the Red Unknown contained Red 3 and Red 40, and the Blue Unknown contains Blue 1 and Red 3. Introduction: Chromatography is an experimental technique used to separate and identify components in mixtures. This technique involves both a stationary and mobile phase. The stationary phase can either involve a liquid or solid. The mobile phase may include either liquid or gas. The mobile phase transports through the stationary phase, carrying some of the mixture with it, causing the two to separate based on its mixture components. Paper chromatography involves the mixture (mobile phase) being placed on the specific chromatography paper (stationary phase). An edge of the paper is then placed into a solvent (acting as mobile phase). The paper draws up the solvent by the capillary action because solvents are attracted to the water molecules on the paper. A sample is applied (spotted) to the origin line of the bottom paper edge, and this edge is placed in the solvent. This causes the solvent to draw up the paper, with the leading edge or solvent front reaching the sample, the components of the mixture attach to the stationary or mobile phase. An established equilibrium exists when each component lies between the two phases, shown with Equation 1: - component mobile phase - coomponent stationary phase
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Chem110 Chromatography Lab Report - Identifying FD&C...

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