MIC+101+Exam+2+Fall+2010

MIC+101+Exam+2+Fall+2010 - MIC 101 Exam 2 Page 1 MIC 101...

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Unformatted text preview: MIC 101 Exam 2 Page 1 MIC 101 Exam 2 (09 November 2010) Please put your Name and Student ID Number on your ScanTron. Please fill-in Form A . Please mark your Answers to Multiple Choice Questions on your ScanTron. Please erase any Mistakes on your ScanTron very Carefully. Please do not ask any Questions during the Exam. Exception: If you think there is a Typo or an Error on any Question, please call me or your TA over. ___________________________________________________________________ 1. A distant Uncle in Shanghai knows you are taking a Microbiology Lab and sends you a really spiffy Shenma Binocular Biological Microscope with no fewer than four (4) Objective Lenses on the Revolving Lens Turret. Golly! To your Surprise, your Biological Microscope has an Ocular Micrometer (!). It is marked from 0 through 100 Ocular Units. You snaffle one of our MIC 101 Stage Micrometers to calibrate your Ocular Micrometer. Using the Yellow- Ringed 10X Lens you find that 25 Ocular Micrometer Units span 100 m. When using the White-Ringed 100X Oil Immersion Lens of your Biological Microscope a Vibrio cholerae measures Twenty (20) Ocular Units. Approximately how large is this Bacterium? A. About 2.0 m. B. About 5.0 m. C. About 8.0 m. D. About 10.0 m. Question 1 Work Space. Please show all Work. You wont get any Credit for this but doing so will build Character and make you a better Person. MIC 101 Exam 2 Page 2 2. Your Idiot Lab Partner obtains the following Data for the Bacterial Growth Curve Experiment: Time (Minutes) OD 0 0.052 10 0.092 20 0.160 30 Not Done 40 0.520 50 0.920 Semi-Log Graph Paper is on Page 3 of your Exam. What is the Doubling Time for this Bacterium? A. 12 Minutes B. 18 Minutes C. 24 Minutes D. 30 Minutes 3. You ve got your Bacteriophage in your 1 Dilution Tube. You notice that the Top Agar Tubes look suspiciously Full. Canasta! Crayola! Your Idiot Lab Partner has measured 6.0 ml of Top Agar to each of the Top Agar Tubes in your 50C Heating Block. What should you do? A. Continue the Experiment. This Set-Up will work and will give Data indistinguishable from the other Clans Data. B. Continue the Experiment. This Set-Up will work if you simply remember to multiply the Number of Plaques you count by 0.5 (Zero Point Five). C. Continue the Experiment. This Set-Up will work if you simply remember to multiply the Number of Plaques you count by 2 (Two). D. Stop the Experiment. This Set-Up will not work. Offer to commit Seppuku for the Honor of your Clan. MIC 101 Exam 2 Page 3 Semi-Log Graph Paper MIC 101 Exam 2 Page 4 4. The other Clan at your Bench has just finished setting-up and labeling all the Tubes necessary for performing the Bacteriophage Titer Experiment. You offer to double-check this Clan s Set-up for them and notice that someone in their Clan has added 9.0 ml Tryptone Broth to each of the Dilution Tubes....
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This note was uploaded on 01/22/2011 for the course MIC 101 taught by Professor Mann during the Fall '09 term at UC Davis.

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MIC+101+Exam+2+Fall+2010 - MIC 101 Exam 2 Page 1 MIC 101...

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