MIC+101+Exam+3+Fall+2010

MIC+101+Exam+3+Fall+2010 - MIC 101 Exam 3 (07 December...

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MIC 101 Exam 3 Page 1 MIC 101 Exam 3 (07 December 2010) • Please put your Name and Student ID Number on your ScanTron. • Please fill-in Form A . • Please mark your Answers to Multiple Choice Questions on your ScanTron. • Please erase any Mistakes on your ScanTron very Carefully. Please do not ask any Questions during the Exam. Exception: If you think there is a Typo or an Error on any Question, please call me or your TA over. ___________________________________________________________________ 1. Your Clan does a Phage Titer Exercise and messes it up Big Time. Your Results are as follows: Plate N 0 . Plaques 10 -4 Confluent 10 -5 Confluent 10 -6 Confluent 10 -7 373 10 -8 Lawn but no Plaques 10 -9 4 What is the most likely Explanation for the Anomaly on your 10 -8 Plate? A. Someone forgot to check the Heating Block Temperature. B. Someone forgot to transfer 500 μ l of the Phage Suspension from the 10 -7 Tube to the 10 -8 Tube. C. Someone forgot to transfer 500 μ l Phage Suspension from the 10 -6 Tube to the 10 -7 Tube. D. Someone forgot to transfer 100 μ l of the Phage Suspension from the 10 -7 Tube to the 10 -8 Agar Overlay.
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MIC 101 Exam 3 Page 2 2. You and your Clan Members jump the Fence of the unspeakably disreputable Mom ʼ s Hot Apple Apartments, swipe a 50 ml Water Sample from the Hot Tub and perform a Most Probable Number (MPN) Test: Tube 1 Tube 2 Tube 3 10 ml (Double-Strength LSL Broth) + + + 1.0 ml (Single Strength LSL Broth) + + + 0.1 ml (Single Strength LSL Broth) - + - These MPN Results indicate that -- A. • The Most Probable Number of Total Bacteria in this Water Sample was 240 Bacteria per Milliliter of Water. • The actual Number (“95% Confidence Limit”) was somewhere between 35 and 470 Bacteria per Milliliter of Water. B. • The Most Probable Number of Total Bacteria in this Water Sample was 240 Bacteria per 100 ml of Water. • The Number of Coliform Bacteria (“95% Confidence Limit”) was somewhere between 36 and 1300 Coliform Bacteria per 100 Milliliters of Water. C. • The Most Probable Number of Coliform Bacteria in this Water Sample was 460 Coliform Bacteria per 100 ml of Water. • The actual Number (“95% Confidence Limit”) was somewhere between 71 to 2400 Coliform Bacteria per 100 ml of Water. D. • The Most Probable Number of Total Bacteria in this Water Sample was 460 Bacteria per Milliliter of Water. • The Number of Coliform Bacteria (“95% Confidence Limit”) was somewhere between 150 to 4800 Coliform Bacteria per Milliliter of Water.
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MIC 101 Exam 3 Page 3 3. You and your Lab Partner set-up a Hemocytometer to determine the Number of Yeast Cells in a Saline Suspension. You obtain the following Data: Medium Square, Upper Right 13 Cells unambiguously inside the Square 03 Cells touching the Top Line 01 Cells touching the Bottom Line 01 Cells touching the Left Line 04 Cells touching the Right Line Medium Square, Upper Left 14 Cells unambiguously inside the Square 04 Cells touching the Top Line
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This note was uploaded on 01/22/2011 for the course MIC 101 taught by Professor Mann during the Fall '09 term at UC Davis.

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MIC+101+Exam+3+Fall+2010 - MIC 101 Exam 3 (07 December...

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