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# exam_fa - Biol 638 Final Exam(Code-1 Biology 638...

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Biol 638, Final Exam (Code-1) 1 Biology 638 Biochemistry II Final Exam (May 14, 2010) You cannot use anything other than your brain, pencils, and eraser in this exam. Using the following values, answer questions in this exam. ATP + H 2 O ADP + P i G°’ = -30 kJ/mol G6P + H 2 O Glucose + P i G°’ = -14 kJ/mol ½O 2 + 2H + + 2 e - H 2 O E°’ = 0.75 V NAD + + H + + 2 e - NADH E°’ = -0.3 V R = 10.0 J·mol -1 · K -1 F = 100.0 kJ·V -1 T = 300 K ln ( x ) = 2 log ( x ) Select the closest answer in all calculations. 1. Assume that the concentrations of ATP, ADP, P i ,H + in the matrix of mitochondrion are constant and are 0.1 mM, 0.01 mM, 0.1 mM, and 10 -8 mM, respectively. The electropotential between the inside and outside is 300 mV (inside is negative). The pH gradient and electropotential energies can be used to synthesize ATP from ADP and P i . What is the pH of outside (intermembrane space) of mitochondrion to maintain the same concentrations of ATP, ADP, P i in the matrix? a. 3 b. 4 c. 5 d. 6 e. 7

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Biol 638, Final Exam (Code-1) 2 2 . O 2 is reduced by NADH to H 2 O in a mitochondrion. If 60% of the all oxidation energy produced in the reaction is utilized to synthesize ATP from ADP + P i , how many ATP would be synthesized from ten H 2 O formation from O 2 and NADH? 3 Assume that G6P is completely oxidized through the pentose phosphate pathway. The produced NADPH is converted to NADH with 66.7% energy efficiency (i.e., 3NADPH = 2NADH). The produced NADH is used to produce ATP from ADP and P i by oxidative phosphorylation. The energy efficiency of the oxidative phosphorylation process is 75%. _____ mole of ATP is produced from one mole of G6P oxidation to CO 2 . a. 20 b. 25 c. 30 d. 35 e. 40
Biol 638, Final Exam (Code-1) 3 4. G6P is synthesized in a biological cell from glucose and ATP using hexokinase. The initial concentrations of glucose, ATP, ADP, and G6P are 0.5 mM, 0.2mM, 0.01 mM, and 0.1 mM, respectively. What is the free energy change (ΔG) kJ/mol in this synthesis? 5 . Assume that one acetyl-CoA is synthesized from CO 2 , H 2 O, and CoA using 20 ATP’s. The produced acetyl-CoA molecules are utilized to synthesize glucose through the glyoxylate pathway and gluconeogenesis. How many ATP molecules are required to make Ten glucose molecules from CO 2 and H 2 O? Assume that NADH, FADH 2 , and GTP are equivalent to three, two and one ATP, respectively. a. 550 b. 600 c. 650 d. 700 e. 750

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Biol 638, Final Exam (Code-1) 4 6 . Assume that a C 3 plant can produce ten G6P using either the cyclic or non-cyclic pathways. Assume that one NADPH is produced from three ATP, but ATP is not produced from
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