exam_1a - Biol 638, Exam-1 (Code-1) Biology 638...

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Biol 638, Exam-1 (Code-1) 1 Biology 638 – Biochemistry II Exam-1 (February 1, 2010) Y cannot use anything other than pencils and eraser in this exam. Using the following values, answer questions 1 - 3. ATP + H 2 O ADP + P i G°’ = -30 kJ/mol Creatine-phosphate + H 2 O Creatine + P i G°’ = -12 kJ/mol ½O 2 + 2H + + 2 e - H 2 O E°’ = 0.80 V FAD + 2H + + 2 e - FADH 2 E°’ = -0.2 V R = 10.0 J·mol -1 · K -1 F = 100.0 kJ·V -1 T = 300 K ln ( x ) = 2 log ( x ) In the calculation questions, select the closest one. 1 . Creatine kinase catalyzes the following reaction. ATP + creatine ADP + creatine-phosphate The G of the above reaction is _______ kJ/mol under the biological standard conditions. a. -40 b. -20 c. -10 d. 20 e. 40 2. pH values of the inside (matrix) and outside (intermembrane space) of mitochondrion are 9 and 5, respectively. The electropotential between the inside and outside is 0.30 V (inside is negative). This electrochemical potential is _______ kJ/mol. a. -10 b. -20 c. -30 d. -40 e. -50 3 . O 2 is reduced by FADH 2 to H 2 O in a mitochondrion by the following reaction: ½O 2 + FADH 2 H 2 O + FAD If the all oxidation energy produced in the above reaction is utilized to synthesize ATP from ADP + P i , how many ATP would be synthesized from five FADH 2 oxidation under standard biological conditions? a. 5 b. 15 c. 20 d. 25 e. 30
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Biol 638, Exam-1 (Code-1) 2 4. Select the statement that is not true. a. Hydrolysis of -phosphate of ATP produces more energetic than that of γ-phosphate. b. Triphosphate moiety of ATP is relatively unstable because its solvation energy is small. c. Triphosphate moiety of ATP is relatively unstable because its resonance stabilization is small. d. ATP occupies the middle rank of phosphoryl-transfer energy production.
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exam_1a - Biol 638, Exam-1 (Code-1) Biology 638...

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