BIOL518 Test #2 2009

BIOL518 Test #2 2009 - Microbial Genetics - BIOL 518 Exam...

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Microbial Genetics - BIOL 518 Exam #2 - Fall 2009 Name: Multiple choice. Choose ALL of the correct answers for each question. There may be more than one correct answer per question. (48 points total). 1: Which of the following is correct regarding oxidation of Guanine: A: Guanine is converted to 8-oxoguanine B: Thymine now pairs with oxidized form of Guanine C: Mutation results in a base transversion D: Mutation results in a base transition 2: Tandem duplications of DNA A: occur as a result of recombination B: typically duplicate small segments of DNA (< 10 bp) C: result in a deletion mutation D: Play an important role in protein functional diversity/evolution 3: In the E. coli SOS response: A: the LexA protein binds directly to single stranded DNA fragments, and cleaves itself to an inactive form. B: the LexA protein, as a dimer, normally functions as a repressor of the genes regulated by the SOS response. C: RecA bound to ssDNA acts as a co-protease for UmuD 2 C resulting in autocleavage of UmuD to form the activated UmuD' 2 C complex. D: Translesion synthesis occurs. 4: Integration of the F plasmid into the E. coli chromosome: A: often occurs by homologous recombination between an IS on F and an IS on the chromosome. B: requires contact with a bacterium that does not contain an F plasmid. C: results in the generation of a high frequency recombination (Hfr) strain. D: can result in formation of an F' plasmid. 5: Which of the following are models of recombination? A: Double Strand Invasion B: Triple Helix Formation C: Double Strand Break Repair D: Single Strand Invasion 6: In the process of Homologous recombination in E. coli : A: homologous alignment of the two DNA's is accomplished by the RecA protein. B: RecA actively promotes the strand exchange of one strand of the original dsDNA for the homologous strand of the ssDNA. C: RecA first binds to a single stranded DNA, and then searches the double stranded DNA for homologous sequences. D: RecA usually requires the action of the RecBCD complex to generate an appropriate substrate for the recombination. 7: The RecBCD complex A: cuts circular plasmid DNA to generate exposed double strand DNA ends B: exhibits 3'-5' exonuclease activty in the absence of a chi site C: generates a 3' single stranded DNA overhang if no chi site is encountered D: requires an additional helicase to separate DNA strands Page 1
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Microbial Genetics - BIOL 518 Exam #2 - Fall 2009 Name: 8: In the RuvABC complex A: RuvC forms planar tetramers that specifically recognizes the structure of the holiday junction B: RuvB forms a hexamer C: RuvB facilitates ATP mediated branch migration D: RuvA is a DNA sequence
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This note was uploaded on 01/22/2011 for the course BIO 518 taught by Professor Heft during the Spring '10 term at Kansas.

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BIOL518 Test #2 2009 - Microbial Genetics - BIOL 518 Exam...

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