Exam 1 2010

Exam 1 2010 - Microbial Genetics BIOL518 Exam 1 - Fall 2010...

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Microbial Genetics BIOL518 Exam 1 - Fall 2010 Name_____________________________________________________________________ Multiple Choice. Choos e all of the correct answers for each question. There may be more than one correct answer per question. (48 points, 4pts each) 1: In the process of Rho-dependent transcription termination: A: Rho protein binds to a stem-loop structure in the mRNA. B: Rho will only bind to mRNA that is being actively translated. C: Rho binds to rut sites in the mRNA. D: it is necessary for RNA polymerase to pause for termination to occur. 2: During translational termination: A: Release factors1 or 2 (RF1 or RF2) bind initially to the P site B: Release factor 3 binds to the Shine-Delgarno C: Release factors 1 or 2 bind to the A site when it is at a nonsense (stop) codon D: Release factor 3 binds to the P site 3: The DNA strand complementary to 5'-AACGCTAGC-3' is: A: 5'-TTGCGATCG-3' B: 5'-AACGCTAGC-3' C: 5'-GCTAGCGTT-3' D: 5'-CGATCGCAA-3' 4: DNA polymerase I functions in DNA replication to: A: synthesize the majority of the DNA. B: join the Okazaki fragments. C: replace the small RNA primers at the 5' end of each Okazaki fragment with DNA. D: unwind the DNA strands at the replication fork. 5: The functions of various regions of E. coli sigma70 are as follows: A: Region 2 recognizes the +1 transcription start site B: Region 3 recognizes the extended -10 sequence at some promoters C: Region 3.2 may block the RNA from exiting RNAP, resulting in abortive initiation D: Region 4 recognizes the -10 sequence 6: Which of the following are true of RNA in bacterial cells? A: Synthesis of mRNA generally uses RNA as the template. B: RNA has important roles in DNA replication and Protein Translation. C: The sugar in RNA is deoxyribose. D: RNA is generally single stranded, but some RNA folds into structures with double stranded regions. 7: In Meselson and Stahl's most famous experiment: A: it was found that DNA replication in E. coli is bidirectional B: it was found that DNA replication occurs by a semiconservative mechanism C: two different isotopes of Nitrogen (15N and 14N) were used to allow separation of "old" and "new" DNA by centrifugation D: DNA of hybrid density was interpreted to consist of one strand each of light and heavy DNA 8: When describing protein structure: A: Primary structure refers to the most stable folded structure of the protein B: Secondary structure refers to the local folding of the protein into alpha-helicies and beta-sheets C: Tertiary structure refers to the interaction between three polypeptide chains to form a trimer D: Quaternary structure refers to the folding of the protein into four consecutive alpha-helicies or four consecutive beta-strands Page 1, v2
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Microbial Genetics BIOL518 Exam 1 - Fall 2010 Name_____________________________________________________________________ 9: In the process of factor independent transcription termination: A: there do not appear to be any proteins or DNA sequences/structures that cause the termination. B:
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Exam 1 2010 - Microbial Genetics BIOL518 Exam 1 - Fall 2010...

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