Exam#1-2009

Exam#1-2009 - Microbial Genetics BIOL518 Exam 1 - Fall 2009...

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Microbial Genetics BIOL518 Exam 1 - Fall 2009 Name_____________________________________________________________________ Multiple Choice. Choos e all of the correct answers for each question. There may be more than one correct answer per question. (52 points, 4pts each) 1: Once DNA polymerase III has completed its role in the replication of the lagging strand: A: the daughter strand is made up of Okazaki fragments which are small RNA segments covalently attached to longer DNA fragments. B: DNA polymerase I uses its 5' to 3' exonuclease activity to remove the RNA primers C: DNA polymerase I uses its 5' to 3' polymerase activity to replace the RNA primers with DNA bps. D: DNA ligase catalyzes the formation of phosphodiester bonds between the adjacent DNA nucleotides in the fragments to form one continuous DNA strand. 2: In the process of Chain Initiation during Transcription in E. coli : A: RNA polymerase requires a primer to begin adding nucleotides. B: a part of the sigma subunit, the 3.2 loop, often initially blocks growth of the RNA chain beyond about 10 nucleotides. C: the RNA polymerse complex with the DNA is referred to as a Closed Complex. D: the first nucleotide of the RNA chain is placed at a position in the DNA template called the +1. 3: E. coli RNA Polymerase containing the sigma-70 subunit: A: uses domain 2 of sigma-70 to specifically recognize the -10 region of a promoter. B: is responsible for transcription of the majority of E. coli genes. C: is referred to as Core RNA Polymerase. D: is made up of a total of five subunits. 4: Which of the following is true of relaxed, B-form DNA? A: There are 10.5 bp per turn of the DNA helix. B: The diameter is 2 nm (which is the same as 20 angstroms). C: It is a right handed helix with a major groove which is deeper and wider than the minor groove. D: The two strands of the DNA are antiparallel, meaning that they have opposite 5' to 3' orientations. 5: The DNA sequences that can be recognized by RNA Polymerase at the beginning of genes include: A: -10 region B: -35 region C: UP element D: extended -10 region 6: Which of the following are true of the Isomerization step of transcription in bacteria? A: The DNA in the vicinity of the promoter region is unwound by RNA Polymerase. B: The "jaws" of RNA Polymerase open relative to RNA Polymerase prior to this step. C: The complex at the end of Isomerization is referred to as an Open Complex. D: The region of unwound DNA is called a replication fork. 7: The enzyme DNA Gyrase: A: can introduce negative supercoils into DNA B: cleaves one strand of the DNA, and passes the other strand through C: can relax positive supercoils in DNA D: requires ATP for its enzymatic activity 8: Mechanisms involved during or immediately following termination of DNA replication of the E. coli chromosome include: A: Interaction between the Tus protein and the DNA Pol III beta clamp to halt replication. B:
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This note was uploaded on 01/22/2011 for the course BIO 518 taught by Professor Heft during the Spring '10 term at Kansas.

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Exam#1-2009 - Microbial Genetics BIOL518 Exam 1 - Fall 2009...

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