Exam#3-2010 - Microbial Genetics BIOL518 Exam 3 - Fall 2010...

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Microbial Genetics BIOL518 Exam 3 - Fall 2010 Name_____________________________________________________________________ Multiple Choice. Choos e all of the correct answers for each question. There may be more than one correct answer per question. (44 points, 4 pts each) 1: When λ phage undergoes a lytic infection: A: specialized transducing phage are likely to result. B: cII activates cI expression. C: Cro activates transcription from the P L and P R promoters. D: the Cro protein represses expression of the cI gene. 2: The phage lambda N protein: A: is required for phage lambda to proceed from very early to early gene expression. B: binds to a seqence on mRNA called a nut site C: is a transcripiton anti-terminator D: modifies RNA polymerase so that it will not recognize transcription terminators 3: The E. coli lacZYA operon has evolved to be very tightly off (very low level of basal transcription) in the absence of lactose. The mechanism that keeps basal transcription very tightly off is: A: LacI protein is degraded in the absence of lactose B: Two LacI dimers bound at lacO sites interacting to form a DNA loop C: CAP protein prevents RNA polymerase from binding to the promoter region D: AsiA protein binds to sigma70, preventing transcription of lacZYA 4: During the process of non-replicative transposition: A: the only DNA replication involved duplicates the target sequence. B: copies of the transposon always end up at both the old and new locations. C: both strands of DNA are cut on both ends of the transposon. D: there is no need for a Resolvase enzyme. 5: In the absence of both glucose and lactose: A: CRP will bind cAMP. B: CRP-cAMP will bind to a site just upstream of the lacZYA promoter (at about -60). C: LacI will bind to allolactose. D: LacI will bind to lacO . 6: When a bacterial cell is competent, it: A: has synthesized all of the structures required for natural transformation B: is capable of transferring the F plasmid to another cell C: has phage DNA integrated into its chromosome D: is capable of taking up free DNA from its surroundings 7: We now know that in the original 1928 experiment, injection of mice with live "rough" (R) and heat killed "smooth" Streptococcus pneumonia cells: A: resulted in the transformation of some of the rough cells, so they became virulent (pathogenic) B: caused the mice to perform better in maze tests C: killed the mice because bacteriophage carried virulence genes from the smooth cells to the rough cells D: the cAMP levels in the rough cells prevented them from being heat killed 8: During natural transformation of Gram positive bacteria, such as Bacillus subtilus , A: a protein fragment (peptide) is used as a signal to induce the state of competence B: transporting the DNA across the outer membrane is a major barrier for DNA uptake C: one strand of the DNA is degraded before transport into the cytosol of the bacterial cell D: DNA is taken up by the bacterial cell through a DNA uptake sequence (DUS) specific mechanism Page 1, v1
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This note was uploaded on 01/22/2011 for the course BIO 518 taught by Professor Heft during the Spring '10 term at Kansas.

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Exam#3-2010 - Microbial Genetics BIOL518 Exam 3 - Fall 2010...

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