FinalExam-2009

FinalExam-2009 - Microbial Genetics BIOL518 Final Exam Fall...

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Microbial Genetics BIOL518 Final Exam - Fall 2009 Name_____________________________________________________________________ Multiple Choice. Choos e all of the correct answers for each question. There may be more than one correct answer per question. (48 points, 4pts each) 1: DNA repair mechansims that can be involved in the repair of pyrimidine dimers include: A: Photoreactivation B: Methyl directed mismatch repair C: Nucleotide excision repair D: SOS response 2: After λ has integrated its DNA to form a lysogen: A: cI repressor must repress expression of all λ genes, except for the cI gene when needed. B: the Q protein is necessary to cause antitermination. C: the SOS response in the host cell can cause the phage to excise and undergo a lytic infection. D: the host protein IHF is necessary to maintain the lysogen. 3: In the process of translation of an mRNA in E. coli : A: the complete 70S initiation complex consists of: 30S and 50S ribosomal subunits; mRNA; and formyl methionine-tRNAfmet. B: charged tRNA's are brought into the A-site by the elongation factor EF-Tu (in a complex with GTP). C: translocation requires the elongation factor EF-G to move the ribosome one codon down the mRNA. D: termination of translation is aided by release factors that bind in the A site. 4: Which of the following statements are true concerning regulation of the araBAD operon? A: The only regulation of araBAD is positive regulation. B: The DNA sites required for fully wild-type regulation of araBAD are: the promoter, araI , araO2 , and the CRP binding site. C: Deletion of araO2 results in 10x higher araBAD expression in the absence of arabinose D: An AraC mediated DNA loop is required to lower the expression of araBAD in the absence of arabinose. 5: Which of the following is true of Specialized Transducing particles? A: They contain only bacterial DNA. B: They are capable of carrying bacterial DNA to a new cell . C: They can be formed by mistakes during the excision of a phage from a lysogenic state. D: Only regions of bacterial DNA that flank the prophage integration site can be found in the particles. 6: Which of the following are processes that can result in a single base mutation? A: Oxidation B: Deamination C: Carbonation D: Mispairing 7: Suppose that each of the below mutations were to occur near the beginning of a gene. Which would have a reasonable chance of expressing a functional protein? A: Deletion of 2 base pairs B: Missense mutation C: Nonsense mutation D: Frameshift mutation Page 1, v1
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Microbial Genetics BIOL518 Final Exam - Fall 2009 Name_____________________________________________________________________ 8: Which of the following are true of protein structure? A:
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This note was uploaded on 01/22/2011 for the course BIO 518 taught by Professor Heft during the Spring '10 term at Kansas.

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FinalExam-2009 - Microbial Genetics BIOL518 Final Exam Fall...

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