Lab6-2011

Lab6-2011 - BIOL 513 Lab 6: Virus characterization Part 4....

Info iconThis preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
BIOL 513 – Lab 6: Virus characterization – Part 4. The nature of virus genome 90 Lab 6: Virus characterization – Part 4. The nature of virus genome – lab designed for 12 groups Equipment for Lab 6: Day 1 Day 2 Day 3 Day 4 Day 5 CO 2 incubator + + + Light microscope - - + Inverted phase contrast microscope + + - Bench-top microcentrifuge - + - Vortex - 6 - Hemacytometer with coverslip - - 12 Hand counter - - 12 2-liter vacuum flask with aspirator 2 2 2 250-mL beaker - 12 - Pipetman, 2-20- µ L 12 - - Pipetman, 40-200- µ L 12 12 12 8-channel Pipetman, 50-300- µ L - - 6 Safety bulb 12 12 12 Rack for microcentrifuge tubes 12 12 12 Rack for 12x75-mm tubes - 24 - Rack for 15-mL tubes 12 - - Rack for 50-mL tubes - 12 12 Supplies & Consumables for Lab 6: Day 1 Day 2 Day 3 Day 4 Day 5 12-well plate cluster - - 12 15-mL sterile conical tube 36 - - 50-mL sterile conical tube - 24 - Sterile 1.5-mL microcentrufuge tube 36 72 12 Sterile 12 × 75-mm tube with snap cap - 576 - Sterile dilution trays - - 12 1-mL pipet - 180 - 5-mL pipet 72 - - 10-mL pipet - - 26 Sterile Pasteur pipet 60 24 6 Sterile filtered tips in trays 12 12 Yellow pipet tips in trays 12 - 12 0.4% Trypan blue stain - - + Crystal violet stain in 50% methanol - - + 70% ethanol in spray bottle 6 6 6 Paper towels - - + KimWipes - - +
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
BIOL 513 – Lab 6: Virus characterization – Part 4. The nature of virus genome 91 Lab 6: Virus characterization – Part 4. The nature of virus genome - lab designed for 12 groups Materials for Lab 6 provided by the instructor: Day 1, Monday, March 7, 2011 CrFk cells, 12-well cluster at ~75-80% confluency – 6 (for 6 supergroups of 2 paired normal lab groups) – These need to be plated on Sunday, March 6th! FCV and FHV in M199+2%FCS, 0.35 mL of each @ 10 6 pfu/mL in 1.5-mL tubes – 12 sets Actinomycin D, 50 µ g/mL in the growth medium, 0.25 mL in 1.5-mL tubes – 12 Fluorodeoxyuridine, 250 µ g/mL (~1 mM) in the growth medium, 0.25 mL in 1.5-mL tubes – 12 CrFk growth medium, 7 mL in 15-mL conical tube – 12 PBS, 7 mL in 15-mL conical tube – 24 Extra CrFk medium Day 2, Tuesday, March 8, 2011 50-mL tube with 50 mL CrFk cell culture medium (DMEM +10% FCS, 1 × AA) – 12 Extra 96-well plate cluster with seeded cells – 6 Extra CrFk medium Day 3, Monday, March 14, 2011 25 mL suspension of freshly trypsinized CrFk cells @ 2-2.5 × 105 cell/mL – 12 (from 3-4 150-mm plates @ 1.8 × 10 7 cells/plate to get total 60-75 × 10 6 cells) Extra CrFk medium
Background image of page 2
BIOL 513 – Lab 6: Virus characterization – Part 4. The nature of virus genome 92 LAB 6 INTRODUCTION TO VIRUS CHARACTERIZATION Determination of Nucleic Acid Type The type of nucleic acid that makes up a viral genome can be determined by a number of methods. In one such method, the replication of a DNA virus is examined in the presence of
Background image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

Page1 / 15

Lab6-2011 - BIOL 513 Lab 6: Virus characterization Part 4....

This preview shows document pages 1 - 4. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online