This preview shows page 1. Sign up to view the full content.
Unformatted text preview: ntrol. As there should not be any bacterial cells in the phage lysate, there should not be any growth on the plate from this control either. If there is growth on this plate, it suggests that either you forgot to filter your lysate through a 0.45 µm filter (remember that the strain the lysate was made on was TetR), or that your filtration was not effective (the filter could have had a hole in it, or you may not have performed the filtration properly). 2 BIOL 519 Week # 8- Generalized Transdu ction 3. 4. 5. 6. 7. As long as there is no growth on either of the two control plates, you can be reasonably sure that any colonies that grow on the plates from tubes 1 and 2 are the result of a transduction reaction. Incubate the tubes containing the transduction reactions at 37oC for 20 min. Add 0.2 ml of 0.1 M sodium citrate to each of the 5 transduction tub...
View Full Document
This note was uploaded on 01/22/2011 for the course BIO 519 taught by Professor Ega during the Spring '10 term at Kansas.
- Spring '10