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Unformatted text preview: t tube. Procedure for P1 transductions: 1. You will be provided with a fresh culture of strain CSH108 that has been grown in LB + CaCl2. The Ca+2 is required for the phage to adsorb to the surface of the bacterial cell to begin the infection process. 2. Set up the following transduction reactions in sterile test tubes: Tube 1 2 3 4 5 CSH108 (ml) 0.1 0.1 0.1 0.1 -P1 lysate (ml) 0.1 0.1 0.1 -0.1 Dilution 100 10-1 10-2 -100 Tubes 1, 2 and 3 are the transduction reactions. Tube 4 is a cells only control. As strain CSH108 is not resistant to , there should be no colonies on the plate from this control. If there are no colonies on this control plate it tells you that the starting strain was truly TetS, and that the plates you used were effective at not allowing growth of TetS strains. Tube 5 is a phage only co...
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This note was uploaded on 01/22/2011 for the course BIO 519 taught by Professor Ega during the Spring '10 term at Kansas.
- Spring '10