Unformatted text preview: plates). • Since does not kill the sensitive cells, but rather inhibits their growth, there are many living TetS cells on your transduction plates. This initial step of patching onto Tet will greatly dilute the TetS cells, and therefore give more reliable results when patching onto Rif and Minimal minus Arg. • If the more than one of the transduction plates has colonies on it, pick the colonies to patch from the plate that was made from the most diluted phage stock that gave colonies. . THURSDAY Fall Break!!! 3...
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This note was uploaded on 01/22/2011 for the course BIO 519 taught by Professor Ega during the Spring '10 term at Kansas.
- Spring '10