Ch_322b_24.05

Ch_322b_24.05 - Analysis of Amino Acids, Polypeptides and...

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Analysis of Amino Acids, Polypeptides and Proteins Van Slyke Determination of Free Amino Groups This classic analysis measures the number of -NH 3 + groups ("free amino groups") with the diazotization reaction that produces N 2 gas that is measured volumetrically. RCHCO 2 - NH 3 + NaNO 2 H 3 O + H 2 O RCHCO 2 - OH + N 2 measure volumetrically on a calibrated vacuum line examples CH 3 CHCH 2 CHCO 2 - NH 3 + CH 3 leucine (0.001 mole) NaNO 2 H 3 O + H 2 O N 2 measure 0.001 mole or 22.4 cc (STP) H 3 NCH 2 CH 2 CH 2 CH 2 CHCO 2 - + NH 3 + lysine (0.001 mole) NaNO 2 H 3 O + H 2 O N 2 measure 0.002 mole or 44.8 cc (STP)
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Automated Amino Acid Analysis Mixtures of amino acids may be separated by a variety of chromatographic methods. One of the most powerful is ion exchange elution chromatography that uses a column of an insoluble polymer of sulfonate groups. The polymer is a modified polystyrene : CH=CH 2 styrene CH-CH 2 -CH-CH 2 -CH-CH 2 polystyrene The modified polystyrene used in ion exchange chromatography is a
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This note was uploaded on 01/23/2011 for the course CHEM 322BL taught by Professor Singer during the Spring '07 term at USC.

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Ch_322b_24.05 - Analysis of Amino Acids, Polypeptides and...

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