topic_11_for_students - Topic 11 Molecular Biology Genetic...

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Topic 11 Molecular Biology
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Genetic Engineering Using in vitro techniques to alter genetic materials in the lab DNA restriction enzymes gel electrophoresis protein and nucleic acid hybridization nucleic acid probes molecular cloning cloning vectors
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Restriction and Modification Enzymes Restriction enzyme: endonuclease that recognizes specific DNA sequences and cuts the phosphate backbone common in prokaryotes rare in eukaryotes used by the cell to protect against invasion by foreign DNA (e.g. viral genomes) - restriction used by researchers for in vitro DNA manipulation
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Restriction Enzymes Three classes; Type II are most commonly studied cleave within their recognition site useful for DNA manipulation Recognize inverted repeats or palindromes typically 4-8 nt long Eco RI: GAATTC
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DNA Restriction restriction enzyme Eco RI Figure 12.1
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Recognition Sites Name: based on species of origin and order of discovery Arrows designate enzymatic cuts * indicates methylation point Py/Pu = pyrimidine/purine
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Restriction Enzymes Restriction enzymes protect cell from foreign
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topic_11_for_students - Topic 11 Molecular Biology Genetic...

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