Lec23 - Lecture 23: Recombinant Click to edit Master...

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Click to edit Master subtitle style Lecture 23: Recombinant Wed 11/17/10 Reading Chapter 20 Pgs 396-408 23-1 Fall 2010, Bio 93, O’Dowd and Warrior, UCI - Copyright: All rights
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Type 1 Diabetes Inheritance: Polygenic disease Environmental factors influence expression Blood glucose level rises Insulin B-cell in pancreas Body cells take up glucose Blood glucose levels fall Blood glucose (90 mg/100 ml) 23-2 Fall 2010, Bio 93, O’Dowd and Warrior, UCI - Copyright: All rights X
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Treatment: Injections of exogenous Insulin Before 1982: insulin purified from animals (pig) 1982: Humulin: synthetic human insulin marketed Produced by recombinant DNA technology More effective Fewer side effects 23-3 Fall 2010, Bio 93, O’Dowd and Warrior, UCI - Copyright: All rights
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DNA cloning in bacteria Ability to combine genes from different sources into a single DNA molecule 23-4 Fall 2010, Bio 93, O’Dowd and Warrior, UCI - Copyright: All rights
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DNA Cloning 1. Make recombinant DNA 2. Transform bacterium (put recombinant plasmid in bacterium) 3. Amplification and selection 23-5 Fall 2010, Bio 93, O’Dowd and Warrior, UCI - Copyright: All rights
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1. Making Recombinant DNA
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Lec23 - Lecture 23: Recombinant Click to edit Master...

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