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Unformatted text preview: 4. Compare and contrast allele-specific cleavage and allele-specific oligonucleotide hybridization. Allele specific cleavage use specific restriction enzyme to recognize specific sequence and cut out normal/bad genes and look at RFLPs to see whether normal or mutant allele is present Allele-specific oligonucleotide hybridization use probes and hybridize to see if it has the normal or mutant allele (use fluorescent markers) 5. Why is it often simpler to treat human genetic disorders at the level of phenotype rather than the level of genotype? Difficult to change DNA in every cell of body...
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This note was uploaded on 01/27/2011 for the course BIOSC 0160 taught by Professor Bledsoe during the Spring '08 term at Pittsburgh.
- Spring '08