BISC%20300.Chap.06.NOTES

BISC%20300.Chap.06.NOTES - 6.4 GROWTH OF CELLS IN BATCH...

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BISC 300 Chapter 6 Notes Microbial Growth 6.1 CELL DIVISION OF BACTERIA AND ARCHAEA Fig. 6.1 Binary transverse fission Fig. 6.2 Budding and polar growth 6.2 POPULATION GROWTH Fig. 6.3 Exponential growth phase Box 6.1 The cell cycle in bacteria Box 6.2 Calculating the generation time Table 6.1 Generation times of several organisms Fig. 6.4 Growth curve Know the growth phases: (1) Lag phase, (2) Exponential (or Logarithmic) phase, (3) Stationary phase, (4) Death or decline phase, and (5) THE FIFTH PHASE = long-term stationary phase or the period of prolonged decrease 6.3 WAYS TO MEASURE GROWTH Total weight of cells Direct count of cells Viability counts Fig. 6.6 Counting the number of viable cells Turbidimetry Fig. 6.8 Relationship between light and absorbency Effect of nutrient concentration on growth rate Table 6.2 Growth yields Fig. 6.10 Relationship between nutrient concentration and total cell mass
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Unformatted text preview: 6.4 GROWTH OF CELLS IN BATCH VERSUS CONTINUOUS CULTURE Batch culture Continuous culture Fig. 6.12 Steady-state relationships 6.5 EFFECT OF ENVIRONMENTAL CONDITIONS ON CELL GROWTH Acidity and alkalinity Fig. 6.13 Effect of pH Presence of oxygen Fig. 6.14 Response of bacterial growth to oxygen availability Toxicity of oxygen Superoxide, hydrogen perodixide, hydroxyl radical, single oxygen Toxicity of O2 and growth of anaerobes Effect of temperature Fig. 6.15 Response of bacterial growth to temperature Fig. 6.16 Growth temperature ranges Table 6.3 Temperature ranges for bacteria and archaea Psychrophiles, mesophiles, thermophiles, hyperthermophiles Temperature limitations CO2 availability Water availability Table 6.4 Tolerance for decreased water activity Fig. 6.17 Optimal salt concentrations...
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